Objective:To duplicate the swine model of left single lung auto-transplantation on purpose to provide the experimental basis for clinical application. Methods:Eighteen healthy local pigs received left pneumonectomy,then the“cutdown” lower lobe was replanted into the thorax with the inferior pulmonary vein transplanted to the proximal stump of the superior pulmonary vein and the bronchus and pulmonary artery anastomosed in turn. They were divided into three groups (6 in each group):group A (non-perfusion fluid treated group),group B (normothermic heparin treated group) and group C (Cold Euro-Collins treated group). The blood sample and lung tissues were collected at baseline,0.5,1,2,and 4 hours after lung auto-transplantation respectively. The indices were determined as follows:the oxygen pressure of pulmonary vein blood,the myeloperoxidase(MPO) content and wet/dry ratio(W/D) of the transplanted lung tissue. The pulmonary grafts were sampled for histological examination and ultra-structure observation under the electronic microscopes. Results:There were no statistical significance of PaO2 levels,MPO content and W/D ratio within the three groups at the time-point of the baseline,0.5,1,or 2 hours after transplantation. Compared with group A,4 hours after transplantation,PaO2 levels,MPO content and W/D ratio had significant difference in group B and C (P < 0.05). Compared with group A,the pulmonary grafts in group B and group C presented less pathological damage and similar degree of injury. Conclusion:This model has satisfactory stability and repeatability. Pulmonary vessel infusion with normothermic heparin meets the requirement of lung auto-transplantation.