Objective:To compare improved‘ceiling’tissue culture method with digestion culture method to choose one method which can culture preadipocytes more properly. Methods:Cells obtained by these two methods,cultured 3 days,were determined by microscope for their self-differentiation. Preadipocytes proliferation and cell cycle were measured by MTT spectrophotometry and flow cytometer. After cultured 9 days,cells were counted to draw growth curves. After induced by DMEM/F12 with insulin,thyrine and transterrin(ITT) 5 days,the diameters of cells were determined by Image-Pro plus5.0 software and the content of lipid was measured by Oil Red O staining. Results:A few adipocytes were obtained by‘ceiling’tissue culture method appeared in the first generation cells. The results of proliferation and differentiation are similar in the cells obtained by the two methods. But ‘ceiling’tissue culture method can obtain more preadipocytes and need less money than digestion culture method. Conclusion:Comared with digestion culture method,improved‘ceiling’tissue culture method is a more simple and economic method which can obtained more preadipocytes