Objective:To understand HIV infection of men who have sex with men(MSMs) in Jangsu Province,and estimate the annual HIV incidence of MSMs. Methods:710 samples from national pilot work of HIV/AIDS comprehensive prevention and care among MSMs in 2008 were tested to find HIV-1 seropositive samples.BED HIV-1 IgG capture enzyme immunoassay(BED-CEIA) was used to find recent HIV-1 infections in HIV-1 seropositive samples,and the annual HIV-1 incidence of MSMs was estimated. Results:40 samples were HIV-1 seropositive and the HIV-1 infection incidence was 5.63%. 23 samples were confimrcl to be recently infected,and the recent infection rate was 57.5%. The corrected annual HIV-1 incidence was 7.54%(95% CI:4.46%~10.62%)among MSMs. Conclusion:The HIV-1 infectin in MSMs in Jiangsu Province has entered a period of rapid growth,powerful prevention and control efforts must be performed.