Objective:To construct early growth response gene-1(Egr-1) and its shRNA expression vectors,and to assess their function on rat glomerular mesangial cell(GMC). Methods:The CDS area of Egr-1 and three reverse repeated motifs targeting of Egr-1 gene were synthesized and cloned into eukaryotic expression plasmid pcDNA3.1-myc-his-A and pGCsi.U6.neo.GFP. After screened and confirmed,the recombinant plasmids were transfected into GMC,then the level of Egr-1 protein in rat GMC was measured by western blot to prove its expression and find out the optimal shRNA. Results:It was verified by partial nucleotide sequencing and restriction endonuclease digestion that the constructed eukaryotic vectors were correct. The results of western blot showed that the constructed pcDNA3.1/Egr-1 plasmid expressed correctly and the optimal shRNA,which effectively silenced the target gene,was Egr-1 shRNA-2. Conclusion:The pcDNA3.1/ Egr-1 plasmid and its shRNA were constructed successfully. These data provide experimental tools for studying biological functions of Egr-1 gene in the future.