Objective:To improve efficiency of transgenes by albumin microbubble,which is a non-virus gene delivery vector. Method:Polyethylenimine coated albumin microbubble(PAMB) was prepared by sonicating the mixture of human albumin,polyethylenimine and glucose. The transgene efficiency of polyethylenimine,polyethylenimine+albumin,PAMB and Lipofectamine 2000 was compared by detecting the positive cell ratio of green fluorescent protein(GFP) using flow cytometry. Results:The average efficiency of transgene by polyethylenimine,polyethylenimine+albumin,PAMB and Lipofectamine 2000 was (13.75±4.88)%,(5.20±1.15)%,(49.17±6.75)% and (53.72±5.69)%,respectively. The efficiency of PAMB group was higher than that in polyethylenimine group(P < 0.05),however,there was no statistical significance between PAMB group and Lipofectamine 2000 group (P > 0.05). Conclusion:Albumin microbubble modified by polyethylenimine is highly effective on transgene,and it is a useful non-virus gene delivery vector. This study may provide a new method for gene transfection in vivo.