Objective:To establish hypoxia model with human umbilical vein endothelial cell line (Eahy926) and investigate the effect of tetramethylpyrazine(TMP) on Eahy926’s fibrinolytic function with hypoxia. Methods:The cells were divided into three groups:control group,CoCl2-treated group (1.6 mmol/L) and CoCl2+TMP-treated groups (0.08 and 0.16 g/L,respectively). Hypoxia was mocked by treatment of CoCl2. Cell viability was evaluated by Hoechst staining and cell counting kit-8 (CCK-8);the expression of t-PA,u-PA and PAI-1 was measured by semi-quantitative RT-PCR;t-PA,u-PA and PAI-1 in the supernatant of culture medium were detected by ELISA. Results:Cell viability was decreased in CoCl2-treated and CoCl2+TMP-treated groups compared with the control group (P < 0.01). Treatment of TMP improved cell viability in a dose-dependent manner (P < 0.01). Moreover,CoCl2 induced a decreasing mRNA expression (P < 0.01) and secretion of t-PA (P < 0.01);TMP treatment elevated t-PA mRNA expression and contents of the cell supernatant (P < 0.01) in a dose-dependent way. Conclusion:TMP can effectively protect Eahy926 cell from hypoxia-induced damage and increase viability of cells in a concentration-dependent manner;TMP might enhance the fibrinolytic function of endothelial cells by regulating the expression and secretion of t-PA.