Objective:To study the expression of GSK3β in chronic allograft nephropathy(CAN)model of rats and investigate the possible role of GSK3β in CAN. Methods:Fisher(F344) kidneys were orthotopically transplanted into Lewis rat recipients to set up chronic allograft nephropathy model in rats. Animals were sacrificed 12 weeks post-transplantation,and the grafts were harvested for histological study,and the expression of GSK3β and NF-κB p65 in renal tissue was analyzed by immunochemistry of SP method. Results:The expression levels of GSK3β and NF-κB p65 in CAN group were significantly higher than those in isograft group and control group. Conclusion:Our research suggests that GSK3β might play an important role in pathogenesis of CAN.