Objective:To construct GIT1-WT(full-length avian GIT1)and GIT1-Y293F lentivirus vectors and to investigate the role of 293 site in GIT1. Methods:The GIT1-WT gene was obtained by polymerase chain traction(PCR)from mouse gene bank. The PCR products were inserted into PLJM-GFP plasmid. The PLJM-GFP-GIT1-Y293F was obtained by TaKaRa MutanBEST Kit. Both PLJM-GFP-GIT1-WT and PLJM-GFP-GIT1-Y293F were evaluated by PCR and sequencing. The virus obtained from transfected 293T cells was infected into osteoblasts. The role of GIT1-Y293F in ostroblasts migration was determined by wound healing assay. Results:The PLJM-GFP-GIT1-WT and PLJM-GFP-GIT1-Y293F were constructed successfully. The results of wound healing assay showed that GIT1-Y293F lentivirus vectors significantly inhibited osteoblast migration compared with GIT1-WT. Conclusion:The functions of GIT1 dependent on the phosphorylation of 293 sites.