Objective:To investigate the relationship between the effect of triptolide on the proliferation of human endometrial carcinoma cell line(HEC-1B)and the apoptosis related signal transduction pathway-PI3K/PKB. Methods:PI3K/PKB pathway inhibitor(LY294002)was used to detect the effect of triptolide on the apoptosis of HEC-1B. The MTT colorimetry method was performed to measure the inhibitive effect of triptolide on cell proliferation;Western blot was used to observe phosphorylated PKB expression. Results:Result of MTT shows PI3K/PKB pathway inhibitor inhibited the function of TP. The inhibitive effect was related to the drug concentration(P < 0.05). Western blot showed the expression of phosphorylated PKB was related to the drug concentration(P < 0.05). Conclusion:Triptolide inhibits the proliferation of HEC-1B. The inhibition effect is related to the PI3K/PKB signal transduction pathway.