Objective:to investigate the effect of platelet-rich plasma(PRP)on human gingival fibroblast function in vitro. Methods:Various concentrations of PRP(1%,5%,10%,20%,30%,50%)were applied to primary cultures of HGFs. Serum containing 10% newborn calf acted as positive control and serum free medium as negative control. MTT assay was utilized to assess proliferation. Migration was determined by assessing the cell response to a concentration gradient with Transwell chamber. Results:PRP had stimulatory effects on the migration of HGFs. A beneficial effect on proliferation was observed,especially in response to 20% PRP. Conclusion:PRP can exert a dose-dependent effect on HGFs proliferation and migration to a certain extent.