Objective:To evaluate the association between the mismatch repair gene hMLH1-93G>A promoter polymorphism and risk of gastric cancer in a population of Jiangsu province in China. Methods:Total of 554 patients with gastric cancer cases and 582 cancer-free controls frequency-matched by age(±5)and sex were recruited in the study. The genotypes of the hMLH1-93G>A polymorphism were detected by TaqMan MGB probe method. We further assessed its association with risk of gastric cancer and interaction with tumor clinic pathological characteristics. Results:There was no significant association of the frequencies of GA or AA among the case and control groups,for case group:GA 262(47.3%),AA 187(33.8%); for control group:GA 269(46.2%),AA 190(32.7%);P=0.636 and 0.398,respectively. The variant genotypes(GA+AA) did not significantly increase the risk of gastric cancer,compared with the GG genotype[adjusted odds ratio(OR)=1.11,95% confidence interval(CI)=0.82~1.51,P=0.487]. No significant association was observed between the variant genotypes of the hMLH1-93G>A polymorphism and the clinic pathological characteristics in gastric cancer. Conclusion:There was no significant association between the hMLH1-93G>A polymorphism and gastric cancer susceptibility.