Objective:To explore the effect of glutamine combined with ulinastatin on intestine damage and inflammatory mediators after severe burn injury. Methods:Total 174 Wistar rats were inflicted with full thickness burns in 30% total body surface area and then randomly divided into burned control (B) group,glutamine treatment (G) group,ulinastatin treatment (U) group and glutamine combined with ulinastatin treatment (G+U) group. Isonitrogenous and isocaloric nutrition supports were provided to all groups. Rats in G group were administered with glutamine at 30% total nitrogen. U group were treated with ulinastation 40 000 IU/kg via tail vein injection. G+U group were given both glutamine and ulinastation. Nitrogen balance,D-lactate,DAO,and the levels of plasma TNF-α and IL-6 were determined. Results:After burn injury,the nitrogen balance,D-lactate,DAO activity,and levels of TNF-α and IL-6 significantly increased. Compared with B group,nitrogen balance in G group was significantly higher,while plasma D-lactate,DAO activity and levels of TNF-α were significantly lower. Levels of TNF-α and IL-6 in U group were significantly lower than those in B group. In G+U group,all the indexes except for nitrogen balance were significantly lower than those in B group. Conclusion:Glutamine combined with ulinastatin treatment may relieve intestine damage after severe burn injury and reduce the level of plasma inflammatory cytokines.