Objective: To investigate the effect of an antiangiogenesis therapy on inhibition of endometrium growth in the nude mouse model. Methods:Recombinant adenovirus was constructed with AdEasy-1 system and AAV 293 cells. Apoptosis of ECV-304 cells was induced by Ad-ES and growth inhibition was observed. The nude mouse endometriosis model was established by subcutaneous implantation. Injected in local focus with Ad-ES,Ad-Track or physiologic saline, the ectopic focuses were detected by microscopy after HE staining. The microvessel densities (MVD) were detected by immunohistochemistry and the apoptosis were detected by TUNEL. Results:The conduction of Ad-ES was proved by PCR, and titer of Ad-ES was 2.06×1010 pfu/ml. The apoptosis of ECV-304 cells induced by Ad-ES was analyzed by flow cytometer and Hoechst 33258 staining. The nude mouse endometriosis model was established by subcutaneous implantation, and the volume of endometriotic lesions treated by Ad-ES was deminished significantly compared with the other two control groups (P < 0.05). MVD and the apoptosis were decreased significantly in the group using Ad-ES compared with the two control groups. Conclusion:ES can induce ECV-304 cells to apoptosis and can inhibit the growth of endometrium in the nude mouse model. Antiangiogenesis may be a potential way for therapy of endometriosis.