Objective: To study the expression and significance of C-erbB-2 and Ki67 in gastric carcinoma. Methods:The expression of C-erbB-2 and Ki67 were examined by SP immunohistochemical technique in 77 cases of primary gastric carcinoma samples. Results:The positive rates of C-erbB-2 in gastric carcinoma and gastritis cases were 62.3% and 6.7%, respectively, a significance existed between the two groups(P < 0.05). The expression rate of C-erbB-2 in gastric carcinoma was positively correlated with the invasion depth in gastric carcinoma(P < 0.05), and was not correlated with age, gender, differentiation and lymph node metastasis(P > 0.05). The positive rate of Ki67 in gastric carcinoma and gastritis cases were 77.9% and 10.0%, respectively, a significance was found between the two groups(P < 0.05). The positive rate of Ki67 in gastric carcinoma was not correlated with age, gender, invasion depth, differentiation and lymph node metastasis(P < 0.05). Conclusion:The over-expression of C-erbB-2 and Ki-67 may be involved in early stage of gastric carcinoma.