Objective: To explore the value of quantitative analysis of contrast-enhanced ultrasonography for diagnosis and differential diagnosis of benign and malignant thyroid nodules. Methods: Sixty-four patients with thyroid nodules who had surgery in our hospital were selected to take contrast-enhanced ultrasonography,and then time-intensity curve(TIC) of contrast-enhanced ultrasonography was analyzed by computer software. Results:①These thyroid diseases which were diagnosed by conventional ultrasound and contrast-enhanced ultrasonography were highly consistent with the pathology results. ②The features of TIC: Most of papillary thyroid carcinomas were “quickly in and slowly out”, most of benign thyroid nodules (including nodular goiters and adenomas) were “simultaneously in and slowly out”. ③Features of quantitative parameters about TIC: The papillary thyroid carcinoma showed “more quickly in, the same intensity and more slowly out”; the nodular goiter showed “simultaneously in, lower intensity and more slowly out”; the adenoma showed “simultaneously in, the same intensity and more slowly out”. The speed of contrast excretion:The papillary carcinoma group was significantly slower than the adenoma group(P < 0.05); the papillary carcinoma group was significantly slower than the benign lesions(P < 0.01). Conclusion: Quantitative analysis of contrast-enhanced ultrasonography is useful in the diagnosis and differential diagnosis of papillary thyroid carcinoma and benign thyroid nodules, and has a good prospect.