Objective:To investigate early diagnosis and treatment of tumor of duodenal papilla. Methods:The clinical data of 63 cases with tumor of duodenal papilla confirmed by operation and pathology from 1992 to 2009 were analyzed retrospectively. Results: The main clinical symptoms were jaundice(41 cases), digestive tract symptoms(18 cases) and hemorrhage of upper digestive tract (4 case). The diagnosis rate of FiScroptic duodcnocndoscopy, endoscopic retrograde cholangio pancreaticography (ERCP), magnetic resonance cholangiopancreatography(MRCP), type-B ultrasonic(BUS) and CT were 90.9%, 100%, 81.8%, 82.5% and 88.1%,respectively. Sixty-one cases underwent Whipple operation and two cases received local resection. Conclusion:ERCP and FiScroptic duodcnocndoscopy are the most effective methods for diagnosis of tumor of duodenal papilla. It is essential to early select radical resection operation so as to improve the result of surgical treatment.