Objective: To investigate the clinical change of cytokines expression in severely burned patients. Methods:Forty-nine burned patients with burn surface exceeding 35% TBSA and healthy controls of same number were enrolled in this study. The peripheral blood of patients were collected at 6 h,12 h and 1,2,7,14 post-burn days. The T lymphocyte proliferation ability was analyzed by CCK-8 and the levels of IL-2-IL-4-IL-6-IL-8 in patients and healthy donors were detected by ELISA. Results:Compared with that in healthy controls,the proliferation ability of T lymphocyte and the levels of IL-2,IL-4 were significantly decreased in patients on 6 h,12 h and 1,2,7,14 post-burn days(all P < 0.05),but the levels of IL-6,IL-8 were significantly higher than those of healthy controls(both P < 0.05). Conclusion:The immunological function of T lymphocyte in severely burned patients is depressed persistently,and the pro-inflammatory cytokines could participate in the pathological process of inflammatory response following burn.