Objective: To investigate the inhibitory activity of anti-latent membrane protein 1(LMP1)antibody fragment (Fab) combined with mitomycin C(MMC) on nasopharyngeal carcinoma NPC-LMP1 xenografts in nude mice. Methods:Nasopharyngeal carcinoma LMP1 cells (5×106) were subcutaneously transplanted in to 20 nude mice, which were randomly divided into Fab group, MMC group, Fab+MMC group and control group. The mice were injected once every 3 days. After corresponding treatments for 5 times, the tumor inhibition rate was evaluated, vascular endothelial growth factor (VEGF) was measured with immunohistochemistry, and the apoptotic rates were analyzed by flow cytometry. Results:The tumor growth inhibition rates in Fab group, MMC group, Fab+MMC group were 18.90%,28.95% and 49.12%,respectively. VEGF in Fab group and Fab+MMC group significantly reduced and showed lower expression compared with control group (P < 0.05). The apoptotic rates in MMC group and Fab+MMC group were (11.84±1.50)% and (17.55±3.21)%, dramatically decreased compared with control group(P < 0.01). Conclusion:Fab and MMC can both inhibit the subcutaneous xenograft tumor growth of NPC LMP1 cells in nude mice with distinct mechanisms. Fab may inhibit the angiogenesis of tumors, while MMC presumably induces apoptosis of tumor cells.