The involvement of apoptosis in vasculogenic mimicry formation of human breast cancer cells
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    Abstract:

    Objective: To investigate the effect of cell apoptosis on the vasculogenic mimicry in human breast cancer cells(MDA-MB-231). Methods:The tube formation ability was assessed using a matrigel basement membrane model, and the structures of the tubular networks were observed under the inverted microscope. Apoptosis cells were stained with Annexin-FITC and PI,and then quantified by fluorescent microscope. Using the broad range caspase inhibitor Z-VAD-FMK and the caspase-3 inhibitor Ac-DEVD-CHO,we further explored the influence of cell apoptosis on forming capillary like structures. Results:MDA-MB-231 cells linked to each other to form wreaths and network structures after 24 h based on three dimensional culture. Capillary-like structures of MDA-MB-231 cells were stained positively with PAS. Apoptosis occurred before capillary-like structure formation but not after capillary-like structures had assembled. Both Z-VAD-FMK and Ac-DEVD-CHO blocked capillary-like structure formation. Conclusion:Breast cancer cells can adopt a capillary-like structure and engage in vasculogenic mimicry. Capillary-like structure formation requires apoptotic cell death through activation of a caspase-dependent mechanism.

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李卫星,孙重期,杜 军.细胞凋亡在人乳腺癌细胞拟态血管形成中的作用[J].南京医科大学学报(自然科学版英文版),2011,(2):179-184.

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  • Received:September 09,2010
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