Objective: To investigate biological activities after CD147 was down-regulated by microRNA in cervical cancer cell line SiHa. Methods:The plasmids pSilencer 4.1-CMV neo/CD147 siRNA was reconstructed, and then they were stablely transfected into SiHa. The expression of CD147,Bcl-2 and caspase-3 were detected by RT-PCR and Western blot assay. Afterwards, the method of MTT was used to analyze the tumor proliferation. The experiment of nude mice was performed to observe the tumor growth. Results:The expression of CD147 mRNA and protein could be inhibited by pSilencer 4.1-CMV neo/CD147 siRNA Sequence 1 and 2. Compared with the control group, cancer cells have more apoptosis and lower proliferation, After cancer cells were transplanted into nude mice,the tumor volume of siRNA groups were smaller than that of the control. Conclusion:The expression of CD147 could be down-regulated effectively by CD147 SiRNA plasmids, the proliferation and the ability of forming tumor could be inhibited evidently after this treatment in cervical carcinoma cells SiHa.