Construction,expression and purification of recombinant H.polyri Tip-α protein
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    Abstract:

    Objective:To construct a recombinant vector containing Tip-α of H.polyri, and transfect it in E.coli to purify recombinant protein. Methods:By PCR technique, Tip-α cDNA was amplified, and then cloned into the pET28a vector. The recombinant plasmid was transformed into E.coli. The correct clone was identified by sequence analysis. The expression product was purified by nick-nitrilotriacetic acid(Ni-NTA) metal-affinity chromatography and analyzed by SDS-PAGE and Western blot methods. Results: Enzyme digestion analysis and sequencing assay showed that Tip-α gene had been successfully inserted into the vector. SDS-PAGE showed a 23 ku protein identified by Western blot analysis. Conclusion:A recombinant plasmid containing Tip-α has been constructed and expressed successfully.

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程文芳,郝 波,张国新,施瑞华.幽门螺杆菌Tipα重组蛋白的表达?纯化及鉴定[J].南京医科大学学报(自然科学版英文版),2011,(2):199-202.

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  • Received:August 24,2010
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