Objective: To investigate the distribution and variety of beta-lactamase genes and ompC gene for outer membrane porin protein C in Escherichia coli (E.coli) strains isolated from urine. Methods:From January 2006 to October 2008, 60 strains of E.coli isolated from urine were collected from The First Affiliated Hospital of NJMU, China. Then, 21 kinds of beta-lactamase genes and ompC gene for outer membrane porin protein C were analyzed by PCR and verified by DNA sequencing. Results: In 60 strains of E.coli, 51 strains (85.0%) , 6 strains(10.0%)and 38 strains(63.3%) were detected to harbor TEM-1-like,OXA-30-like and CTX-M-55-like by DNA sequencing, respectively. Moreover, 54 strains were detected mutaions in ompC gene for outer membrane porin protein C (90.0%), and 6 strains among them were confirmed as new mutation types by BLASTn. But other 6 strains(10.0%) were detected no mutaion in ompC gene. Conclusion:Both CTX-M-55-like beta-lactamase gene and ompC gene for outer membrane porin protein C play a key role in resistance to beta-lactam antimicrobial agents. Among 6 strains of E.coli containing new mutation types in ompC gene for outer membrane porin protein C, 3 strains combined with co-existing TEM-1-like and CTX-M-55-like beta-lactamase genes, 2 strains combined with co-existing TEM-1-like beta-lactamase gene, 1 strain combined with co-existing TEM-1-like and OXA-30-like beta-lactamase genes.