Objective: To quantify the volume and function of atrium in the patients with atrial septal defect(ASD) with full volume three-dimensional echocardiography(FV3DE) and evaluate the influence of occluder closure on the volume and function of atrium. Methods:FV3DE data of 43 patients with ASD and 40 normal controls were acquired and analyzed. Minimum volume (Vmin), maximum volume (Vmax) and pre-contraction volume (Vp) of atrium were measured, and the reservoir function, conduit function and pump function were calculated. All the results were compared respectively. Results:Vmin,Vp, Vmax, active emptying volume (Vae),passive emptying volume(Vpe)and total emptying volume(Vte)of right atrium in the patients with ASD were larger than those in the controls(P < 0.05); While passive emptying fraction (Vpef),active emptying fraction (Vaef) and total emptying fraction (Vtef)were smaller than those in the controls significantly(P < 0.05). After the occluder closure of ASD, the Vmin,Vp,Vmax,Vae,Vaef,Vpe,Vte and Vtef of right atrium became smaller, while Vp, Vmin, Vmax of left atrium became larger that before. And the Vaef, Vpef, Vte, Vtef of left atrium became smaller than those before operation. Conclusion:The atrial volume and function in the patients with ASD can be evaluated with FV3DE. As for the patients with ASD, reservoir function and conduit function of the right artrium increases and pump function decreases. After occluder closure, the volume of right atrium was retrieved, volume of left atrium enlarges for compensation, while the conduit function and pump function of both atria reduce.