Objective: To explore the method of cryopreservating human induced pluripotent. Methods:Colonies of induced pluripotent stem cells(iPS cells) were dissected into pieces(containing approximately 100~200 cells) using mechanical methods, then sequentially treated with 10%,20% vitrification solution, and sealed in mini straws with the second solution. Mini straws were then dropped into liquid nitrogen immediately for vitrification and cryopreservation. After vitrificated preservation, iPS cells were thawed in 37℃ water bath, and were immediately balanced in 0.2 mol/L sucrose solution and then in 0.1 mol/L sucrose solution, and at last plated on feeder layer cells. Alkaline phosphatase activity, expressions of OCT4, SOX2 in iPS cells and cell differentiation were evaluated. Results:Post vitrification and thawing, iPS cells maintain properties of pluripotent stem cells, including normal morphology, alkaline phosphatase staining,OCT4 and SOX2 expression. Calculation of colony recovery rates indicates that approximate(77.40±13.12)% of iPS cell colonies survived the freezing and thaw procedures. Conclusion:Vitrification with ministraws is a very useful and effective cryopreservation method for iPS cells.