objective:to investigate the effect of silencing cd147 expression on proliferation and invasion of human laryngeal carcinoma cell line hep2. methods:previously prepared recombinant plasmids psilencer-shrna-control, psilencer-shrna1 and psilencer-shrna2 were transfected into the hep2 cell. the rna interference efficiency of cd147 was determined by real-time pcr and western blot. the proliferation was measured by mtt assay. the transwell test was used to detect the invasion ability of the hep2 cells, and psilencer-shrna-control transfected hep2 and normal hep2 were used as control groups. results:after the transfection with psilencer-shrna1 and psilencer-shrna2, the mrna and protein levels of cd147 in the hep2 cells were both significantly down-regulated. proliferation was decreased significantly to 67.49%(p < 0.01)and 61.37%(p < 0.01),respectively. their invasive ability were also significantly decreased to 61.80%(p < 0.01)and 73.58%(p < 0.01),respectively. conclusion:the shrna down-regulated the expression of cd147, inhibited the proliferation and invasive ability of hep2 cells.