Objective:To explore the positive rate and expression levels of p53 and c-erbB-2 in gastric cancer and their relationship with tumor invasion. Methods:SP immunohistochemistry was used to detect the positive rates and expression of p53 and c-erbB-2 expressed in 139 gastric cancer with different invasive depth of lamina propria(T1a),submucosa(T1b),muscularis propria(T2) and suserosa(T3). Results:The positive rates of p53 in T1a and T1b tumors were lower than T3 tumors significantly(P < 0.05),but the expression levels of p53 hadn′s significant difference among tumors with different invasive depth(P < 0.05). The positive rates and expression levels of c-erbB-2 augmented with increased invasive depth of tumor(P < 0.01). The positive rates of c-erbB-2 were related with p53 (P < 0.05),but the expression levels of c-erbB-2 and p53 hadn′t significant relationship (P > 0.05). Ordinal logistic regression analysis showed that both intensity expression of p53 and low, medium, high degree of c-erbB-2 expression affected the invasive depth of gastric cancer significantly(P=0.001,P=0.010,P=0.000,P=0.000,respectively). Conclusion:The expression of p53 and c-erbB-2 in gastric cancer are not synchronous and p53 mutation contributes to c-erbB-2 expression, but the expression level of c-erbB-2 is not related with p53. The mutation of anti-oncogene p53 maybe related to the origin of gastric cancer,while the invasion of gastric cancer is mostly relate to activation of oncogene c-erbB-2.