objective:to observe the effect of electro-acupuncture(ea) on serum testosterone(t) and spermatogenesis in normal rests at different developmental stages. methods:sprague-dawley rats were assigned into four groups:juvenile group (jg),early-puberty group (epg),later-puberty group (lpg)and adult group(ag). each group was subdivided into four teams:ea at acupoints(ewa),ea at non-acupoints(ena),immobilization control(ic),normal control(nc). ea(3 hz)at ewa or ena was performed for 20 min per day in different developmental group of rats for 10 days. sperm number was counted and serum level of t was measured after ea treatment. results:the results showed that the serum level of t in the epg,lpg and ag rats were significantly depressed(p < 0.05). sperm counts in the epg,lpg and ag groups were also significantly reduced. conclusion:the results suggested that repeated stimulation with ea is a good option to reduce the serum t and sperm quantity of male rats after the puberty period.