objective:to analyze the relationship of seropositive rate of hbsag with hepatitis b vaccination in a randomly selected general population in three counties of jiangsu province,and improve the comprehensive control against hepatitis b. methods:with cluster random sampling in different regions,questionnaire survey was conducted and blood samples were collected to detect hbsag by elisa. results:the standardized seropositive rates of hbsag were 4.61%,6.88% and 10.06% in zhangjiagang,danyang and taixing respectively,and the seropositive rate of hbsag among people over 18 years old who were born before programmed immunization started was significantly higher than that among people under 18 years old. the hepatatis b vaccine inoculation rates were 39.56%,19.66% and 41.04% in zhangjiagang,danyang and taixing respectively. in the population over 18 years,the seropositive rate of hbsag among people who were inoculated with hepatitis b vaccine was lower than that among people who hadn’t been inoculated. conclusion:the programmed hepatitis b immunization of newborns has got great achievement and hepatitis b vaccine inoculation among adults also obtained notable results. the immunization for the adults with hepatitis b vaccine should be improved to better control the infection of hbv.