Objective:To assess the migration and differentiation capacity of β-galactosidase(β-gal) transgenic mouse derived amniotic mesenchymal stem cells (AMSCs) in recipient wild type mice after the transplantation subcutaneously. Methods:AMSCs were isolated from amnion membrane of the second-trimester β-gal transgenic mice and were cultured in either α-MEM containing 15% FBS, osteogenic- or adipogenic-induced medium. The second passage AMSCs derived from β-gal transgenic mice were transplanted subcutaneously into newborn C57BL/6 wild type mice. After 8 weeks,various organs from recipient mice were fixed and stained for Lac Z or embedded in paraffin. Then 5-μm sections were cut on a rotary microtome. The paraffin sections were dewax and observed under microscopy, and stained by immunofluorescence for β-gal,glial fibrillary acidic protein (GFAP) and myelin basic protein(MBP). Results:The second passage AMSCs derived from β-gal transgenic mice were positive for Lac Z staining and could differentiate into osteoblasts or adipocytes under cultures with the osteogenic- or adipogenic-induced medium. Followed subcutaneous injection of the second passage AMSCs derived from β-gal transgenic mice into wild type mice for 8 weeks,Lac Z positive cells were detected in thyroid,lung,spleen and brain of recipient wild type mice.The double positive cells for β-gal and GFAP, or β-gal and MBP, were detected in brain of recipient wild type mice. Conclusion:AMSCs transplanted through subcutaneously can survive at least 2 months,and migrate into multiorgans through circulation and differentiate into organ specific cells to participate in self-renewal of normal tissues. Our results indicate that AMSCs can be used as seed cells for stem cell therapy to treatment various diseases.