Objective:To study the protective effects of JNK(the c-Jun NH-terminal kinase)inhibitor and NAC (N-acetylcysteine) on neuronal apoptosis induced by oxyen-glucose deprivation/regain in vitro. Methods:The oxygen-glucose deprivation/regain (OGD/R) models were established in vitro. The neurons were exposed to OGD 4h before being placed into normal culture medium. Group A was taken as a blank control. Spinal cord neurons received the JNK inhibitor and NAC treatment before regaining the oxygen-glucose in group B and C,respectively. The neuronal apoptosis were detected by MTT assay in different time points(4 h,12 h,24 h after regaining oxygen-glucose). Results:The model of OGD/R in spinal cord neurons was successfully established. Cell viability in MTT assay were higher in group B and group C than that in group A, and there was no significant difference between group B and group C. Conclusion:The JNK inhibitor and NAC can effectively protect neurons from apoptosis induced by OGD/R.