Objective:To investigate the curative effect of ultrasound-mediated transdermal drug delivery combined with compound ossotide on treatment of fracture. Methods:Forty mature New Zealand rabbits were prepared and the 3mm-long bone defects were made in both radii of each rabbit as fracture model. Right limbs belong to experimental groups (therapy groups) and left limbs belong to control groups. X-ray imaging,observation of light microscope and morphometry of osseous tissue were performed to assess the degree of fracture healing in both groups at the end of 2 th,4 th,6 th and 8 th week postoperatively. Results:All results showed that callus formation and bone linking in experimental gruops were much earlier than that in control groups(P < 0.05). Conclusion:The ultrasound-mediated transdermal drug delivery combined with compound ossotide has a positive effect on the whole process of fracture healing.