Objective:To study the different expression of miRNA in plasma between gastric cancer patients and healthy people for finding new biomarkers. Methods:The levels of 7 miRNAs (miR-21,-27a,-191,-379,-658,-433,-383) in plasma samples from patients of gastric cancer and healthy controls were measured using real-time quantitative RT-PCR. Results:miR-191 and miR-27a both elevated significantly in gastric cancer patients’ plasma(P < 0.001). miR-191 yields an AUC(the area under the ROC curve) of 0.939, with 86.1% sensitivity and 100% specificity in discriminating gastric cancer from controls. miR-27a yields an AUC of 0.881, with 83.33% sensitivity and 86.67% specificity. Conclusion:miR-191 and miR-27a are potential novel biomarkers in detection of gastric cancer.