Objective: To compare the arterial spin labeling (ASL) and dynamic susceptibility-weighted contrast-enhanced(DSC)magnetic resonance (MR) imaging and explore the clinical application of ASL technique in the pre-operation of gliomas. Methods: Twenty-three patients with gliomas(all the cases were verified histologically,including 17 patients of high-grade gliomas,6 patients of low-grade gliomas) were routinely examined by 3T MRI including ASL and DSC before operation. The maximal cerebral blood flow of solid regions of tumor (TBFmax) and the cerebral blood flow (CBF) of opposite white matter,grey matter and hemisphere were measured on maps. Results: All of 23 cases demonstrated consistency between ASL and DSC MR imagings. The ratio of TBFmax/opposite white matter CBF,TBFmax/opposite grey matter CBF,TBFmax/opposite hemisphere CBF revealed no statistically significant difference between the two techniques. But there were statistical differences between high-grade and low-grade gliomas. Conclusion: The noninvasive and repeatable ASL technique was almost as sensitive as DSC MRI in detecting brain gliomas perfusing abnormalities. It is helpful to give the gradation of gliomas before operation.