Objective: To evaluate the value of transrectal contrast-enhanced ultrasound(CEUS) in the differential diagnosis of prostate begin and malignant nodules. Methods: Thirty-three patients with 38 nodes were examined with transrectal ultrasound(TRUS) and CEUS. And then nodule-target TRUS guided biopsy was performed on those nodules. After biopsy,datum of CEUS were replayed and time intensity curves(TIC) of regions of interest(ROI) were analyzed with auto tracking contrast quantification(ACQ)software. Several parameters were measured on each nodule. Statistic analyses of all data were performed between the groups divided by pathologic results. Results: Twenty of 38 nodules were benign and the other 18 ones were malignant. Most of prostate cancer nodules showed increased enhancement(14/18). However,the benign nodules mainly showed equal enhancement(14/20). The average time to peak (TTP) and accelerating time (ACT) of malignant nodule,(26.03± 3.12) s and (8.22± 3.07) s respectively,were shorter than those of benign nodule,(30.98± 7.15) s and (12.26± 5.30) s,respectively(P < 0.05). And peak intensity (PI) of prostate cancer nodule,(20.43± 5.32) dB,was lower than that of benign lesion,(23.51± 2.51) dB,P < 0.05. The difference of two groups had statistical significance. Conclusion: CEUS could reveal the presence of vasculature within the lesions of prostate cancer objectively. TTP,ACT and PI could help to discriminate prostate malignant lesions from benign ones.