The effect of toughening genes with ester key on targeting to tumor cells of curcumin
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    Abstract:

    Objective: Produced curcumin’s precursors with toughen genes with ester key,to observe its different effect on growing cells between prostates’ and the normal diploid’s. Methods: Human prostatic cancer DU-145 cells were cultured and incubated with Boc-phenylalanine-curcumin (BPC) for 6-24 h. Cell viability was detected by MTT. Cell apoptosis rate was detected by flow cytometry. DU-145 cells were cultured and incubated with BPC for 1-7 d for cell grow curve detected by counting process. The people aortic smooth muscle cells(HASMC) acted as control. Results: After treatment with 10~40 μmol/L BPC for 6-24 h,the growth inhibition of DU-145 cells was 7.37%~66.87%(P < 0.05),in dose-time-dependent manners. Partial cancer cells presented morph-ological characters of apoptosis,with FSC-SSC scatter diagram. After treatment for 24 h,the cell apoptosis rate was 37.84%~47.12%(P < 0.05). Compared with curcumin of same concentrations,the control group HASMC apoptosis rate was 0.94%~4.23%(P < 0.05). Conclusion: The prodrug of curcumin could induce apoptosis of prostatic cancer DU-145 cells,and reduce the side effects on normal diploid cells. This provided a novel strategy for further exploration of tumor-targeted chemotherapeutic drugs.

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任德帅,陈方敏,石家齐,严 波,汤 磊,杨 燕,常傲霜,李登宝,肖 锋.酯键链接增韧基团增强姜黄素对肿瘤细胞靶向性作用[J].南京医科大学学报(自然科学版英文版),2011,(10):1403-1406.

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  • Received:May 08,2011
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