Objective: To study the influence of hyperbilirubinemia upon newborns central nervous system by pass multiple-frequency auditory steady-state evoked responses analysis. Methods: Clinical data and examination results of multiple-frequency auditory steady-state evoked responses (ASSR) of 280 cases,blood total bilirubin consistency (TBC) higher than 220.6 μmol/L(12.9 mg/dl) month newborns,were retrospectively reviewed. Results: One hundred and ninty-five cases with TBC less than 310.0 μmol/L,had normal ASSR;18 cases with TBC higher than 520.0 μmol/L all took place encephalpathy of bilirubin,among them,16 cases of ASSR were heavy degree abnormal;67 cases with TBC less than 519.9 μmol/L or higher than 310.1 μmol/L,had no encephalopathy clinical performance,but ASSR appeard an abnormality,among them,63 cases (94.0%) were light-mid-degree abnormal,2 cases were heavy degree abnormal. The abnormality degree of ASSR is related with TBC,TBC and albumin ratio(TB/A)(P < 0.01),but wasn’t related to the constituent of pathogenesis of hyperbilirubinemia(P > 0.05). Conclusion: ASSR can suggest preclinical nervous poisonsing of hyperbilirubinemia in early stage,when TBC is higher than 307.8 μmol/L,ASSR should be included as a normal test.