Objective:To investigate the mechanism of deoxypodophyllotoxin(DOP) against the peripheral nervous system. Methods: The effects of DOP on voltage-dependant K+ channels were studied by using whole-cell patch-clamp recordings on acute isolated rat dorsal root ganglion(DRG) neurons. Results: DOP can reversibly block voltage-dependent potassium current(IK) and delayed-rectifier potassium current(IKDR) and make the curve of the current-voltage relation shift to positive potentials at the concentration of 10,20,40 and 80 μmol/L. This study thus demonstrated that DOP is a dose-dependant and reversibly blocker of IK and IKDR in DRG neurons. The inhibition rate of DOP on IKDR was higher than on IK. Conclusion: It can be concluded that the effect of DOP on the current of potassium channel of DRG neurons may be a possible contributing mechanism for the toxin on peripheral nervous system.