Objective: To observe the effects of silencing of Livin gene expression on the apoptosis of MG-63 osteosarcoma cells. Methods: Double stranded RNA targeting the Livin gene was chemically synthesized in vitro and transfected into MG-63 osteosarcoma cells by LipofectamineTM 2000. The transfection efficiency was observed by fluorescence confocal microscopy. Reverse transcription-polymerase chain reaction(RT-PCR) and Western blot were used to detect the expression of Livin at mRNA and protein levels. Apoptosis analysis was performed by annexin V staining. Results: The Livin mRNA expression of the Livin-siRNA transfected group was 0.195 ± 0.019,significantly lower than those of blank control(0.539 ± 0.031) and the negative control group(0.438 ± 0.029). The Livin protein expression of the Livin-siRNA transfected group was 0.165 ± 0.022,significantly lower than those of blank control(0.632 ± 0.034) and the negative control group (0.625 ± 0.035). Increased apoptotic cells were observed by flow cytometry. Conclusion: siRNA can inhibit Livin expression of MG-63 osteosarcoma cells and induce cell apoptosis. Livin might serve as a target for apoptosis-inducing therapy of osteosarcoma.