Objective: To investigate the differencial expression of miRNA-26a in allergic asthma children and murine asthma model. Methods: Expressions of miRNA-26a in allergic asthma children and ovalbumin-induced mouse asthma model were analyzed by real-time PCR. The number of cells in the bronchoalveolar lavage fluid (BALF) of murine asthma model was acessed. The expression of GSK-3β in murine asthma model was acessed by Western blot. Results: miRNA-26a was up-regulated in allergic asthma children and murine asthma model compared with normal controls. The total cell number and acidocyte number in BALF from murine asthma model were significant higher than control. Enhanced eosinophilic inflammation in lung was observed in murine asthma model. Protein level of GSK-3β in murine asthma model was lower than control. Conculsion: miRNA-26a expression level was higher in allergic asthma children and murine asthma model than controls.