Objective: To observe the clinical effects in patients with biliary tract cancer treated with OLF (L-OHP,LV,5-Fu) combined with hydroxycamptothecin(HCPT) which was administrated as intraperitoneal perfusion hyperthermia. Methods: Twenty-four patients were given chemotherapy as well as intraperitoneal chemotherapy combined with hyperthermia. The curative effects were estimated according the criterea of RECIST and the clinical benefit response rate(CBR) was evaluated. The CBR refers to pain,physical status and body weight. Results: There was no complete response among all the patients. Partial response was observed in 7 patients(29.2%) and stable disease was 33.3%. As to CBR,16 patients of total(66.7%) achieved the clinical benefit response and their quality of life was improved. The toxicities were marrow depression and alimentary tract reactions. Conclusion: This thermo-chemotherapy comprehensive regimen was an effective and safty methods for patients with advanced biliary tract cancer.