Objective: To investigate the effect of ankle joint function rehabilitation therapy for cerebral function reconstruction and paralysis limbs function in patients with acute cerebral infarction(ACI) using functional magnetic resonance imaging (fMRI). Methods: Before and after treatment,BOLD-fMRI examinations were performed by 1.5T MRI to 11 ACI patients with rehabilitation therapy,5 ACI patients without rehabilitation therapy respectively. Five healthy volunteers act as controls. The block stimulation function testing was introduced to observe the changes in cerebral activated areas and activated volume and Fugl-Meyer movement function scores. Results: The areas activated by healthy side ankle joint of ACI patients moving were similar to the normal group. When paralyzed side ankle joint moving,in addition to contralateral cerebral hemisphere region(SM1),the ipsilateral SM1 and PMC areas were also activated in varying degrees. The activated volume in the contralateral hemisphere is larger than that in the ipsilateral volume(P < 0.05). After treatment,the activated volume in the contralateral SM1 region was increased obviously(P < 0.05). The activated volume of patients with rehabilitation therapy was more significantly increased(P < 0.05). The Fugl-Meyer movement score was increased after treatment,and the increase of score was more significant in the patients with rehabilitation therapy,compared to the group without treatment(P < 0.01). Conclusion: The fMRI can display objective reconstruction feature of brain after ACI. Early stage moving function rehabilitation therapy of ankle is beneficial to reconstruction of cerebral function and recovery of paralysis limbs function.