Objective: To explore the relationship between the methylenetetrahydrofolate reductase (MTHFR) gene C677T and A1298C polymorphism and unhealthy gravidity history. Methods: Fifty-three women with unhealthy gravidity history and fifty-seven controls were genotyped for MTHFR gene C677T and A1 298C polymorphism using sequencing technique. Results: In the control group,there was 3 of 677T/T genotype and 9 of 677C/T genotype meanwhile 1 298A/C genotype;in the experiment group,there was 9 of 677T/T genotype and 18 of 677C/T genotype meanwhile 1 298A/C genotype. There was an obvious difference between the experiment and the control group in the frequency 677T/T genotype,and 677C/T genotype with 1 298A/C genotype (P < 0.05). Conclusion: MTHFR C677T and A1 298C mutation allele may be related to the increased risk of unhealthy gravidity history.