Objective: To survey the prevalence of HIV-1 drug resistance and subtype distribution among population with newly infected with HIV who were younger than 25 years of age and not received antiretroviral treatment. Methods:Blood specimens of HIV infected individuals were collected and HIV-1 pol gene was sequenced. The genetic mutations associated with drug resistance were identified with Stanford HIV Drug Resistance Database online. The phylogenetic tree was built with MEGA4.0 software,and subtypes were analyzed. Results: No mutations associated with HIV-l drug resistance was found in the reverse transcriptase and protease region. There are 4 subtypes,CRF01_AE,B,CRF07_BC and CRF08_BC,identified among 47 isolates. CRF01_AE was predominated,which account for 59.6%,followed by B subtype which reached to 25.5%. Conclusion: The first line regiments of national anti-retrovirus treatment is still effective. Testing drug resistance to HIV is not needed before starting anti-retroviral therapy(ART). Subtype distribution of HIV changed obviously compared with 2006. To further know dynamic HIV prevalence in Jiangsu province,the molecular epidemiology study on HIV-infected population confirmed within the past two years should be conducted.