Objective:To elucidate whether Ginsenosides-Rbl (Gs-Rb1) improved myocardial remodeling and whether Gs-Rb1 improved myocardial fibrosis related protein/mRNA in rat heart with chronic heart failure(CHF) induced by adriamycin. Methods:CHF models of rats were performed by adriamycin. CHF rats were randomly divided into CHF group (n = 15) and Gs-Rb1 group(n = 17) that received 70 mg/(kg·d) Gs-Rb1. In addition,age-matched health rats were referred as control group(n = 10). At the 4th week,the heart to body weight (HW/BW) and the left ventricular to body weight (LW/BW) was analyzed by echocardiography. Histology was used to assess myocardial interstitial fibrosis in the heart. The expression of transforming growth factor β1 (TGF-β1),connective tissue growth factor (CTGF) and endothelin 1 (ET-1) were detected by Western blot and RT-PCR. Results: Compared to CHF group,Gs-Rb1 significantly improved HW/BW(P = 0.005) and LW/BW(P = 0.000);The protein expression of TGF-β1,CTGF and ET-1 were more significantly down-regulated in Gs-Rb1 group than in CHF group,which in those both group were significantly higher than those in control group(P < 0.05);The mRNA expression of TGF-β1,CTGF and ET-1 were more significantly down-regulated in Gs-Rb1 group than in CHF group,which in the both group were significantly higher than in control group(P < 0.05). Conclusion:The effects of Gs-Rb1,on improving myocardial remodeling in CHF rats,were fulfilled by increasing protein and mRNA of TGF-β1,CTGF and ET-1.