Objective: To investigate the expression pattern of microRNA(miRNA) in pancreas of 2 type diabetes rats treated with ramipril by miRNA array technique. Methods: Male Spmgue-Dawley (SD) rats were injected in peritoneal with streptozocin (40 mg/kg) and fed with high sugar and fat for 1 weeks,then treated with ramipril [50 mg/(kg·d)] for 28 days. When the rats were sacrificed,total RNA was extracted from myocardium and the differential expressions of miRNA were detected by miRNA array. Results: Contrast to control group rats,the expression level of 27 miRNAs changed significantly in ramipril group rats,20 of them was up-regulated and the other 7 were down-regulated. Conclusion: The expression profile of miRNA changes in pancreas of 2 type diabetes rats treated with ramipril,which implies that those changed miRNAs may play an important role in improvement of pancreas function in SD rats.