Objective: To investigate the distribution of acquired resistance-related genes and markers of mobile genetic elements,and their relationships in pandrug-resistant Acinetobacter baumannii(PDR-ABA). Methods: From March 2010 to May 2010,20 strains of PDR-ABA were collected from clinical specimen. Then,57 kinds of acquired resistance-related genes against beta-lactams,aminoglycosides,quinolones,and 8 kinds of genetic markers of mobile genetic element were analyzed by PCR. The index cluster analysis was used to investigate their relationships. Results: In 20 strains of PDR-ABA,5 kinds of acquired beta-lactam-resistance genes,4 kinds of acquired aminoglycoside-resistance genes,1 kinds of acquired quinolone-resistance genes,and 5 kinds of genetic markers of mobile genetic elements were detected. Index cluster analysis showed that acquired resistance-related genes were associated with mobile genetic elements. TEM-1,ADC-like,OXA-23,aac(6′)-Ⅰb,ant(2＂)-Ⅰ,ant(3＂)-Ⅰ,aphA1 and adeB had close relations with tnpU,ISCR1,IS26,ISaba1;DHA-1 and CARB also had relations with IS903. Conclusion: In this group of PDR-ABA,acquired resistance-related genes played an important role in resistant phenotypes of antimicrobial agents,and acquired resistance-related genes were associated with mobile genetic elements.