Objective:To investigate resistance to pathogens isolated from blood culture of the First Affiliated Hospital of Nanjing Medical University in 2010,which can be used to guide clinical therapy. Methods: Total 3 625 blood culture samples were collected from this hospital in 2010. Bacteria and fungus were identified by API systems;bacterial drug sensitivity was determined by disk diffusion method;drug sensitivity of fungi were determined by Rosco method;and Whonet 5.5 software was used for data analysis. Results: Among the 3 625 blood culture samples,419 cases showed positive. Gram-positive bacteria accounted for 57.8%,Gram-negative bacteria accounted for 38.4%,and fungi accounted for 3.8%. The resistance analysis showed that there was no resistant strain to vancomycin and teicoplanin in Staphylococcus and Enterococcus. Enterobacteriaceae isolates were highly sensitive to carbapenem. Non-fermenting bacteria showed a relatively high resistance to many antimicrobial drugs. Conclusion: There are many different kinds of pathogens isolated from blood culture,which showed high resistance to many antimicrobial agents.