Objective: To evaluate the energy metabolism and mitochondrial function of skeletal muscle in type 2 diabetic patients offspring using dynamic 31P magnetic resonance spectroscopy(31P-MRS). Methods: Dynamic 31P-MRS was performed on 21 type 2 diabetes patients offsprings and 18 healthy volunteers. Spectra were acquired from quadriceps muscle while subjects were at rest,exercise and recovery. The peak area of inorganic phosphate (Pi),phosphocreatine (PCr) and adenosine triphosphate (ATP) were measured. The concentration of adenosine diphosphate (ADP) and intracellular pH value were calculated from the biochemistry reaction equilibrium. The time constant and recovery rate of PCr,Pi and ADP were analyzed using exponential curve fit. Results: There were no significance differences of Pi,PCr,ADP,β-ATP and the ratio of Pi/PCr between the type 2 diabetes patients offsprings and the healthy volunteers at rest. The type 2 diabetes patients offsprings showed significantly lower β-ATP concentration at the end of exercise compared to the healthy subjects. At recovery phase,the PCr and β-ATP concentration and pH of the type 2 diabetes patients offsprings were lower than those of the healthy subjects. The ratio of Pi/PCr of the type 2 diabetes patients offspring is significantly higher than that of the healthy subjects at the third spectrum of recovery phase. The mean PCr recovery rate of the offspring is significantly slower than that of the healthy subjects. Conclusion: Noninvasive 31P-MRS could detect the energy metabolism inefficiency and mitochondrial function impairment in skeletal muscle of type 2 diabetes patients offsprings.