Objective:To investigate the expression of glucagon like peptide 1 receptor(GLP-1R) in mouse bone marrow mesenchymal stem cells(BM-MSCs) transfected by adenovirus and the effect of Exendin-4/GLP-1R on BM-MSCs proliferation and apoptosis after transfection. Methods: The adenoviral vector containing GLP-1R gene was constructed through the Ad.Max-system and infected the BM-MSCs. The expression of GLP-1R in Ad.GLP-1R-BM-MSCs was examined by RT-PCR,immunofluorescence,and Western blot,respectively. The effects of Exendin-4/GLP-1R on cell growth,cell cycle and apoptosis in Ad.GLP-1R-BM-MSCs were evaluated by cell counting and flow cytometry. Results: BM-MSCs could be effectively infected by adenovirus. When multiplicity of infection (MOI) was 400, the infection efficiency was 89.5%. Mouse BM-MSCs didn’t express the GLP-1R gene natively. After infected with Ad.GLP-1R,the BM-MSCs expressed the GLP-1R gene and protein effectively,and the cell growth could be stimulated by Exendin-4. Compared with the unstimulated Ad.GLP-1R-BM-MSCs,G0/G1 phase cells in the Exendin-4 stimulation group decreased significantly,G2/M phase cells and proliferation index (S+G2/M) increased significantly (P < 0.01). But there was no significant difference in the apoptosis rate of two groups. Conclusion: Ad.GLP-1R can infect mouse BM-MSCs effectively and induce the expression of GLP-1R. Exendin-4 can promote the proliferation of BM-MSCs infected with Ad.GLP-1R.