Objective:To synthesize the uncoupler 2-[(1H-benzo[d][1,2,3]triazol-5-ylamino) methyl]-4,6-dichlorophenol for neuronal nitric oxide synthase(nNOS) with postsynaptic density protein-95(PSD-95). Methods: 5-amine-1H-benzo[d][1,2,3]triazol was firstly synthesized from 4-nitrobenzene-1,2-diamine by diazotization and reduction. Then the target compound was obtained by reduction after condensation of 5-amine-1H-benzo [d][1,2,3]triazol and 3,5-dichloro-2-hydroxybenzaldehyde. Rusults: The target compound was confirmed by hydrogen nuclear magnetic resonance spectra (1H-NMR) and carbon nuclear magnetic resonance spectra (13C-NMR),and the overall yield is 20.3%. Conclusion: The program of experiments can be used to synthesize 2-[(1H-benzo[d][1,2,3]triazol-5-ylamino) methyl]-4,6-dichlorophenol quickly and easily.